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MAP1LC3B polyclonal antibody

  • Catalog # : PAB29556
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial synthetic protein of human MAP1LC3B.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to amino acids 1-30 at N-terminus of human MAP1LC3B.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 14.7
  • Reactivity:
  • Human, Rat
  • Form:
  • Liquid
  • Purification:
  • Ammonium sulfate precipitation
  • Isotype:
  • Ig
  • Recommend Usage:
  • Immunofluorescence (1:100)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of Lane 1: Nontransfected 293 cell lysates (2 ug/lane), Lane 2: Transiently transfected 293 cell lysates (2 ug/lane) with MAP1LC3B polyclonal antibody (Cat # PAB29556) at 1:1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of Chloroquine (50 uM, 16h) treated (A) and untreated (B) U251 cells with MAP1LC3B polyclonal antibody (Cat # PAB29556) at 1:100 dilution.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Gene Information
  • Gene Name:
  • MAP1LC3B
  • Gene Alias:
  • LC3B,MAP1A/1BLC3
  • Gene Description:
  • microtubule-associated protein 1 light chain 3 beta
  • Gene Summary:
  • The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq
  • Other Designations:
  • microtubule-associated proteins 1A/1B light chain 3
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