MAP1LC3B polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against partial synthetic protein of human MAP1LC3B.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 1-30 at N-terminus of human MAP1LC3B.
Host
Rabbit
Theoretical MW (kDa)
14.7
Reactivity
Human, Rat
Form
Liquid
Purification
Ammonium sulfate precipitation
Isotype
Ig
Recommend Usage
Immunofluorescence (1:100)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Nontransfected 293 cell lysates (2 ug/lane), Lane 2: Transiently transfected 293 cell lysates (2 ug/lane) with MAP1LC3B polyclonal antibody (Cat # PAB29556) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunofluorescence
Immunofluorescent staining of Chloroquine (50 uM, 16h) treated (A) and untreated (B) U251 cells with MAP1LC3B polyclonal antibody (Cat # PAB29556) at 1:100 dilution. -
Gene Info — MAP1LC3B
Entrez GeneID
81631Gene Name
MAP1LC3B
Gene Alias
LC3B, MAP1A/1BLC3
Gene Description
microtubule-associated protein 1 light chain 3 beta
Gene Ontology
HyperlinkGene Summary
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq
Other Designations
microtubule-associated proteins 1A/1B light chain 3
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