Western blot analysis of Lane 1: Nontransfected 293 cell lysates (2 ug/lane), Lane 2: Transiently transfected 293 cell lysates (2 ug/lane) with MAP1LC3A polyclonal antibody (Cat # PAB29555) at 1:1000 dilution.
Immunofluorescent staining of Chloroquine (50 uM, 16h) treated U251 cells with MAP1LC3A polyclonal antibody (Cat # PAB29555) shows positivity in cytoplasmic autophagic vacuoles at 1:200 dilution.
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq