MAPK15 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MAPK15.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human MAPK15.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of MAPK15 polyclonal antibody (Cat # PAB2954) in T-47D cell line lysates (35 ug/lane). MAPK15 (arrow) was detected using the purified polyclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of MAPK15 (arrow) using rabbit MAPK15 polyclonal antibody (Cat # PAB2954). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK15 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MAPK15 polyclonal antibody (Cat # PAB2954) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — MAPK15
Entrez GeneID
225689Protein Accession#
NP_620590;Q8TD08Gene Name
MAPK15
Gene Alias
ERK7, ERK8
Gene Description
mitogen-activated protein kinase 15
Gene Ontology
HyperlinkOther Designations
OTTHUMP00000189334|extracellular regulated kinase 8 delta|extracellular signal regulated kinase 8|extracellular signal-regulated kinase 8
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Interactome
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Publication Reference
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ERK8 down-regulates transactivation of the glucocorticoid receptor through Hic-5.
Saelzler MP, Spackman CC, Liu Y, Martinez LC, Harris JP, Abe MK.
The Journal of Biological Chemistry 2006 Jun; 281(24):16821.
Application:IHC-P, Human, Human lung .
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Activation of the Erk8 mitogen-activated protein (MAP) kinase by RET/PTC3, a constitutively active form of the RET proto-oncogene.
Iavarone C, Acunzo M, Carlomagno F, Catania A, Melillo RM, Carlomagno SM, Santoro M, Chiariello M.
The Journal of Biological Chemistry 2006 Feb; 281(15):10567.
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Characterization of the reversible phosphorylation and activation of ERK8.
Klevernic IV, Stafford MJ, Morrice N, Peggie M, Morton S, Cohen P.
The Biochemical Journal 2006 Feb; 394(Pt 1):365.
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ERK8 down-regulates transactivation of the glucocorticoid receptor through Hic-5.
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