GATA1 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with GATA1 polyclonal antibody (Cat # PAB29506).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human bone marrow with GATA1 polyclonal antibody (Cat # PAB29506) shows distinct nuclear positivity in bone marrow poietic cells.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human GATA1.
Immunogen
Recombinant protein corresponding to human GATA1.
Sequence
EGLDAAASSTAPSTATAAAAALAYYRDAEAYRHSPVFQVYPLLNCMEGIPGGSPYAGWAYGKTGLYPASTVCPTREDSPPQAVEDLDGKGSTSFLETLKTERLSPDLLTLGPALP
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:20-1:50)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with GATA1 polyclonal antibody (Cat # PAB29506).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human bone marrow with GATA1 polyclonal antibody (Cat # PAB29506) shows distinct nuclear positivity in bone marrow poietic cells. -
Gene Info — GATA1
Entrez GeneID
2623Gene Name
GATA1
Gene Alias
ERYF1, GF-1, GF1, NFE1
Gene Description
GATA binding protein 1 (globin transcription factor 1)
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. [provided by RefSeq
Other Designations
GATA binding protein 1|NF-E1 DNA-binding protein|OTTHUMP00000023259|erythroid transcription factor 1|globin transcription factor 1|transcription factor GATA1
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Interactome
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Disease
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