TCF3 polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human testis with TCF3 polyclonal antibody (Cat# PAB29500) shows strong nuclear positivity in a subset of cells in seminiferus ducts.
Immunofluorescence
Immunofluorescent staining of MCF-7 cells with TCF3 polyclonal antibody (Cat# PAB29500) under 1-4 ug/mL working concentration shows positivity in nucleus but excluded from the nucleoli. Antibody staining is shown in green.
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Specification
Product Description
TCF3 polyclonal antibody raised against recombinant human TCF3.
Immunogen
Recombinant protein corresponding to amino acids of human TCF3.
Sequence
RTFSEGTHFTESHSSLSSSTFLGPGLGGKSGERGAYASFGRDAGVGGLTQAGFLSGELALNSPGPLSPSGMKGTSQYYPS
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human testis with TCF3 polyclonal antibody (Cat# PAB29500) shows strong nuclear positivity in a subset of cells in seminiferus ducts.Immunofluorescence
Immunofluorescent staining of MCF-7 cells with TCF3 polyclonal antibody (Cat# PAB29500) under 1-4 ug/mL working concentration shows positivity in nucleus but excluded from the nucleoli. Antibody staining is shown in green. -
Gene Info — TCF3
Entrez GeneID
6929Protein Accession#
P15923Gene Name
TCF3
Gene Alias
E2A, ITF1, MGC129647, MGC129648, bHLHb21
Gene Description
transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
Omim ID
147141Gene Ontology
HyperlinkGene Summary
The TCF3 gene, also called E2A, encodes 2 basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. E12 and E47 are involved in regulation of immunoglobulin gene expression (Bain et al., 1994 [PubMed 8001125]).[supplied by OMIM
Other Designations
E2A immunoglobulin enhancer-binding factor E12/E47|immunoglobulin transcription factor 1|kappa-E2-binding factor|transcription factor 3|transcription factor E2-alpha
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