MDFI polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human placenta with MDFI polyclonal antibody (Cat# PAB29499) shows moderate cytoplasmic positivity in trophoblastic cells.
Immunofluorescence
Immunofluorescent staining of A-431 cells with MDFI polyclonal antibody (Cat# PAB29499) under 1-4 ug/mL working concentration shows positivity in nucleus but excluded from the nucleoli. Antibody staining is shown in green.
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Specification
Product Description
MDFI polyclonal antibody raised against recombinant human MDFI.
Immunogen
Recombinant protein corresponding to amino acids of human MDFI.
Sequence
LPNDSGHPSELGGTRRAGNGALGGPKAHRKLQTHPSLASQGSKKSKSSSKSTTSQIPLQAQEDCC
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human placenta with MDFI polyclonal antibody (Cat# PAB29499) shows moderate cytoplasmic positivity in trophoblastic cells.Immunofluorescence
Immunofluorescent staining of A-431 cells with MDFI polyclonal antibody (Cat# PAB29499) under 1-4 ug/mL working concentration shows positivity in nucleus but excluded from the nucleoli. Antibody staining is shown in green. -
Gene Info — MDFI
Entrez GeneID
4188Protein Accession#
Q99750Gene Name
MDFI
Gene Alias
I-MF, I-mfa
Gene Description
MyoD family inhibitor
Omim ID
604971Gene Ontology
HyperlinkGene Summary
This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. [provided by RefSeq
Other Designations
OTTHUMP00000016375|OTTHUMP00000196414|inhibitor of MyoD family a
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