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BRD4 polyclonal antibody

  • Catalog # : PAB29479
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  • Specification
  • Product Description:
  • BRD4 polyclonal antibody raised against recombinant human BRD4.
  • Immunogen:
  • Recombinant protein corresponding to amino acids of human BRD4.
  • Sequence:
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunofluorescence (1-4 ug/mL)
    Immunohistochemistry (1:200-1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human skin with BRD4 polyclonal antibody (Cat# PAB29479) shows strong nuclear positivity in epidermal cells.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of PC-3 cells with BRD4 polyclonal antibody (Cat# PAB29479) under 1-4 ug/mL working concentration shows positivity in nucleus but excluded from the nucleoli. Antibody staining is shown in green.
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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  • Gene Information
  • Gene Name:
  • BRD4
  • Gene Alias:
  • Gene Description:
  • bromodomain containing 4
  • Gene Summary:
  • The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq
  • Other Designations:
  • bromodomain-containing 4,bromodomain-containing protein 4,chromosome-associated protein
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