BRD4 polyclonal antibody
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human skin with BRD4 polyclonal antibody (Cat# PAB29479) shows strong nuclear positivity in epidermal cells.
Immunofluorescence
Immunofluorescent staining of PC-3 cells with BRD4 polyclonal antibody (Cat# PAB29479) under 1-4 ug/mL working concentration shows positivity in nucleus but excluded from the nucleoli. Antibody staining is shown in green.
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Specification
Product Description
BRD4 polyclonal antibody raised against recombinant human BRD4.
Immunogen
Recombinant protein corresponding to amino acids of human BRD4.
Sequence
PPFIATQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSN
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (1:200-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human skin with BRD4 polyclonal antibody (Cat# PAB29479) shows strong nuclear positivity in epidermal cells.Immunofluorescence
Immunofluorescent staining of PC-3 cells with BRD4 polyclonal antibody (Cat# PAB29479) under 1-4 ug/mL working concentration shows positivity in nucleus but excluded from the nucleoli. Antibody staining is shown in green. -
Gene Info — BRD4
Entrez GeneID
23476Protein Accession#
O60885Gene Name
BRD4
Gene Alias
CAP, HUNK1, HUNKI, MCAP
Gene Description
bromodomain containing 4
Omim ID
608749Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq
Other Designations
bromodomain-containing 4|bromodomain-containing protein 4|chromosome-associated protein
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