Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon with GAR1 polyclonal antibody (Cat# PAB29476) shows strong nuclear positivity in glandular, endothelial and ganglion cells at 1:500 - 1:1000 dilution.
Immunofluorescent staining of U-2 OS cells with GAR1 polyclonal antibody (Cat# PAB29476) under 1-4 ug/mL working concentration shows positivity in nucleus and nucleoli. Antibody staining is shown in green.
This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA2 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. These four H/ACA snoRNP proteins are also components of the telomerase complex. The encoded protein of this gene contains two glycine- and arginine-rich domains and is related to Saccharomyces cerevisiae Gar1p. Two splice variants have been found for this gene. [provided by RefSeq
nucleolar protein family A member 1,nucleolar protein family A, member 1,nucleolar protein family A, member 1 (H/ACA small nucleolar RNPs)