POLD3 polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human stomach tissue with POLD3 polyclonal antibody (Cat# PAB29475) shows strong nuclear positivity in glandular cells.
Immunofluorescence
Immunofluorescent staining of U-251 MG cells with POLD3 polyclonal antibody (Cat# PAB29475) under 1-4 ug/mL working concentration shows positivity in nucleus. Antibody staining is shown in green.
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Specification
Product Description
POLD3 polyclonal antibody raised against recombinant human POLD3.
Immunogen
Recombinant protein corresponding to amino acids of human POLD3.
Sequence
SVTEPKLATPAGLKKSSKKAEPVKVLQKEKKRGKRVALSDDETKETENMRKKRRRIKLPESDSSEDEVFPDSPGAY
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human stomach tissue with POLD3 polyclonal antibody (Cat# PAB29475) shows strong nuclear positivity in glandular cells.Immunofluorescence
Immunofluorescent staining of U-251 MG cells with POLD3 polyclonal antibody (Cat# PAB29475) under 1-4 ug/mL working concentration shows positivity in nucleus. Antibody staining is shown in green. -
Gene Info — POLD3
Entrez GeneID
10714Protein Accession#
Q15054Gene Name
POLD3
Gene Alias
KIAA0039, MGC119642, MGC119643, P66, P68
Gene Description
polymerase (DNA-directed), delta 3, accessory subunit
Omim ID
611415Gene Ontology
HyperlinkGene Summary
The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA; MIM 176740), the multisubunit replication factor C (see MIM 102579), and the 4 subunit polymerase complex: POLD1 (MIM 174761), POLD2 (MIM 600815), POLD3, and POLD4 (MIM 611525) (Liu and Warbrick, 2006 [PubMed 16934752]).[supplied by OMIM
Other Designations
DNA polymerase delta, subunit 3|polymerase (DNA directed), delta 3
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Interactome
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Pathway
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Disease
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