CMPK1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human CMPK1.
Immunogen
Recombinant protein corresponding to human CMPK1.
Sequence
NLQGWNKTMDGKADVSFVLFFDCNNEICIERCLERGKSSGRSDDNRESLEKRIQTYLQSTKPIIDLYEEMGKVKK
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Immunofluorescence (1-4 ug/mL)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Human cell line RT-4 Lane 2: Human cell line U-251MG sp Lane 3: Human plasma (IgG/HSA depleted) Lane 4: Human liver tissue with CMPK1 polyclonal antibody (Cat # PAB29453) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human uterus with CMPK1 polyclonal antibody (Cat # PAB29453) shows strong cytoplasmic positivity in glandular cells at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line MCF7 with CMPK1 polyclonal antibody (Cat # PAB29453) at 1-4 ug/mL concentration shows positivity in nucleus but excluded from the nucleoli. -
Gene Info — CMPK1
Entrez GeneID
51727Gene Name
CMPK1
Gene Alias
CMK, CMPK, UMK, UMP-CMPK, UMPK
Gene Description
cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
Omim ID
191710Gene Ontology
HyperlinkGene Summary
Uridine monophosphate (UMP)/cytidine monophosphate (CMP) kinase (EC 2.7.4.4) catalyzes the phosphoryl transfer from ATP to UMP, CMP, and deoxy-CMP (dCMP), resulting in the formation of ADP and the corresponding nucleoside diphosphate. These nucleoside diphosphates are required for cellular nucleic acid synthesis (Liou et al., 2002 [PubMed 11912132]).[supplied by OMIM
Other Designations
Cytidine monophosphate kinase|OTTHUMP00000009565|UMP-CMP kinase|cytidylate kinase
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Interactome
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Pathway
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