TRIP6 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human TRIP6.
Immunogen
Recombinant protein corresponding to human TRIP6.
Sequence
RPGSLDAEIDLLSSTLAELNGGRGHASRRPDRQAYEPPPPPAYRTGSLKPNPASP
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Immunofluorescence (1-4 ug/mL)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Human cell line RT-4 Lane 2: Human cell line U-251MG sp with TRIP6 polyclonal antibody (Cat # PAB29450) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human kidney with TRIP6 polyclonal antibody (Cat # PAB29450) shows strong cytoplasmic and membranous positivity in cells in tubules and cells in glomeruli at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with TRIP6 polyclonal antibody (Cat # PAB29450) at 1-4 ug/mL concentration shows positivity in plasma membrane, cytoplasm and focal adhesion sites. -
Gene Info — TRIP6
Entrez GeneID
7205Gene Name
TRIP6
Gene Alias
MGC10556, MGC10558, MGC29959, MGC3837, MGC4423, OIP1, ZRP-1
Gene Description
thyroid hormone receptor interactor 6
Omim ID
602933Gene Ontology
HyperlinkGene Summary
This gene is a member of the zyxin family and encodes a protein with three LIM zinc-binding domains. This protein localizes to focal adhesion sites and along actin stress fibers. Recruitment of this protein to the plasma membrane occurs in a lysophosphatidic acid (LPA)-dependent manner and it regulates LPA-induced cell migration. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq
Other Designations
OPA-interacting protein 1|thyroid receptor-interacting protein 6|zyxin related protein 1
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