PARP2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human PARP2.
Immunogen
Recombinant protein corresponding to human PARP2.
Sequence
ARALNESKRVNNGNTAPEDSSPAKKTRRCQRQESKKMPVAGGKANKDRTEDKQDESVKALLLKGKAPVDPECTAKVGKAHVYCEGNDVYDVMLNQTNLQFNNNKYYLIQLLEDDAQRNFSVWM
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Immunofluorescence (1-4 ug/mL)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of U-2 OS with PARP2 polyclonal antibody (Cat # PAB29444) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with PARP2 polyclonal antibody (Cat # PAB29444) shows moderate nuclear positivity in cells in seminiferus ducts at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line MCF7 with PARP2 polyclonal antibody (Cat # PAB29444) at 1-4 ug/mL concentration shows positivity in nucleus. -
Gene Info — PARP2
Entrez GeneID
10038Gene Name
PARP2
Gene Alias
ADPRT2, ADPRTL2, ADPRTL3, PARP-2, pADPRT-2
Gene Description
poly (ADP-ribose) polymerase 2
Omim ID
607725Gene Ontology
HyperlinkGene Summary
This gene encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq
Other Designations
ADP-ribosyltransferase (NAD+; poly(ADP-ribose) polymerase)-like 2|poly (ADP-ribose) polymerase family, member 2|poly (ADP-ribosyl) transferase-like 2|poly(ADP-ribose) synthetase
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Disease
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