NLK polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of NLK polyclonal antibody (Cat # PAB2944) in A-375 cell lysate. NLK (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with NLK polyclonal antibody (Cat # PAB2944) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NLK.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human NLK.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of NLK polyclonal antibody (Cat # PAB2944) in A-375 cell lysate. NLK (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with NLK polyclonal antibody (Cat # PAB2944) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — NLK
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Interactome
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Pathway
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Disease
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Publication Reference
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Nemo-like kinase induces apoptosis in DLD-1 human colon cancer cells.
Yasuda J, Tsuchiya A, Yamada T, Sakamoto M, Sekiya T, Hirohashi S.
Biochemical and Biophysical Research Communications 2003 Aug; 308(2):227.
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Regulation of lymphoid enhancer factor 1/T-cell factor by mitogen-activated protein kinase-related Nemo-like kinase-dependent phosphorylation in Wnt/beta-catenin signaling.
Ishitani T, Ninomiya-Tsuji J, Matsumoto K.
Molecular and Cellular Biology 2003 Feb; 23(4):1379.
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The TAK1-NLK mitogen-activated protein kinase cascade functions in the Wnt-5a/Ca(2+) pathway to antagonize Wnt/beta-catenin signaling.
Ishitani T, Kishida S, Hyodo-Miura J, Ueno N, Yasuda J, Waterman M, Shibuya H, Moon RT, Ninomiya-Tsuji J, Matsumoto K.
Molecular and Cellular Biology 2003 Jan; 23(1):131.
Application：IP-WB, Human, 293, PC12 cells.
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Nemo-like kinase induces apoptosis in DLD-1 human colon cancer cells.
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