ABHD12B polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human ABHD12B.
Immunogen
Recombinant protein corresponding to human ABHD12B.
Sequence
LGTGVATNAAKVLEEKGCPVDAIVLEAPFTNMWVASINYPLLKIYRNIPGFLRTLMDALRKDKIVFPNDENVKFLSSPLLILHGEDDRTVPLEYGKKLYEIARNAYRNKERVKMVIFPPGFQHNLLCKSPTLLITVRDFLSKQ
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:200-1:500)
Immunofluorescence (1-4 ug/mL)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate) Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with ABHD12B polyclonal antibody (Cat # PAB29379) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with ABHD12B polyclonal antibody (Cat # PAB29379) shows strong nuclear positivity in cells of seminiferus ducts at 1:200-1:500 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with ABHD12B polyclonal antibody (Cat # PAB29379) at 1-4 ug/mL concentration shows positivity in nucleus but excluded from the nucleoli. -
Gene Info — ABHD12B
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