Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla) with SOD1 polyclonal antibody (Cat # PAB29364) at 1:250-1:500 dilution.
Immunohistochemical staining of human liver with SOD1 polyclonal antibody (Cat # PAB29364) shows nuclear and cytoplasmic positivity in hepatocytes at 1:2500-1:5000 dilution.
Immunofluorescent staining of human cell line U-251 MG with SOD1 polyclonal antibody (Cat # PAB29364) at 1-4 ug/mL concentration shows positivity in cytoplasm and nucleus but excluded from the nucleoli.
The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq
Cu /Zn superoxide dismutase,Cu/Zn superoxide dismutase,SOD, soluble,indophenoloxidase A,superoxide dismutase, cystolic