DNAJA1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla) with DNAJA1 polyclonal antibody (Cat # PAB29361) at 1:250-1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with DNAJA1 polyclonal antibody (Cat # PAB29361) shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts at 1:20-1:50 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with DNAJA1 polyclonal antibody (Cat # PAB29361) at 1-4 ug/mL concentration shows positivity in cytoplasm and microtubules.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human DNAJA1.
Immunogen
Recombinant protein corresponding to human DNAJA1.
Sequence
DQKDHAVFTRRGEDLFMCMDIQLVEALCGFQKPISTLDNRTIVITSHPGQIVKHGDIKCVLNEGMPIYRRPYEKGRLIIEFKVNFPENGFLSPDKLSLLEKLLPERKEVEETDEMDQVELVDFDPNQERRRHYNGE
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:20-1:50)
Immunofluorescence (1-4 ug/mL)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla) with DNAJA1 polyclonal antibody (Cat # PAB29361) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with DNAJA1 polyclonal antibody (Cat # PAB29361) shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts at 1:20-1:50 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with DNAJA1 polyclonal antibody (Cat # PAB29361) at 1-4 ug/mL concentration shows positivity in cytoplasm and microtubules. -
Gene Info — DNAJA1
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Interactome
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Disease
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