NMT2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human NMT2.
Immunogen
Recombinant protein corresponding to human NMT2.
Sequence
GGTKSDSASDSQEIKIQQPSKNPSVPMQKLQDIQRAMELLSACQGPARNIDEAAKHRYQFWDTQPVPKLDEVITSHGAIEPDKDNVRQEPYSLPQGFMWDTLDLSDAEVLKELYTLLNENYVEDDDNMFRFDYSPEFLLWALRPPGWLL
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:500-1:1000)
Immunofluorescence (1-4 ug/mL)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Human cell line RT-4 with NMT2 polyclonal antibody (Cat # PAB29360) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with NMT2 polyclonal antibody (Cat # PAB29360) shows strong cytoplasmic positivity in cells in seminiferus ducts at 1:500-1:1000 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with NMT2 polyclonal antibody (Cat # PAB29360) at 1-4 ug/mL concentration shows positivity in plasma membrane, cytoplasm and the Golgi apparatus. -
Gene Info — NMT2
Entrez GeneID
9397Gene Name
NMT2
Gene Alias
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Gene Description
N-myristoyltransferase 2
Omim ID
603801Gene Ontology
HyperlinkGene Summary
N-myristoyltransferase (NMT) catalyzes the reaction of N-terminal myristoylation of many signaling proteins. It transfers myristic acid from myristoyl coenzyme A to the amino group of a protein's N-terminal glycine residue. Biochemical evidence indicates the presence of several distinct NMTs, varying in apparent molecular weight and /or subcellular distribution. The predicted 498-amino acid of human NMT2 protein shares 77% and 96% sequence identity with human NMT1 and mouse Nmt2 comprise two distinct families of N-myristoyltransferases. [provided by RefSeq
Other Designations
OTTHUMP00000019188|glycylpeptide N-tetradecanoyltransferase 2
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Interactome
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Disease
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