SH3BP1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human SH3BP1.
Immunogen
Recombinant protein corresponding to human SH3BP1.
Sequence
ENLSNLRYLMKFLARLAEEQEVNKMTPSNIAIVLGPNLLWPPEKEGDQAQLDAASVSSIQVVGVVEALIQSADTLFPGDINFNVSGLFSAVTLQDTVSDRLASEELPSTAVPTPATTPAPAPAPAPAPAPALASAATKERTESEVPP
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:20-1:50)
Immunofluorescence (1-4 ug/mL)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Human cell line RT-4 with SH3BP1 polyclonal antibody (Cat # PAB29348) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human colon with SH3BP1 polyclonal antibody (Cat # PAB29348) shows cytoplasmic positivity in glandular cells at 1:20-1:50 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with SH3BP1 polyclonal antibody (Cat # PAB29348) at 1-4 ug/mL concentration shows positivity in cytoplasm. -
Gene Info — SH3BP1
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Interactome
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