MKI67 polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human lymph node with MKI67 polyclonal antibody (Cat # PAB29347) shows strong nuclear positivity in a fraction of cells.
Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with MKI67 polyclonal antibody (Cat # PAB29347) at 1-4 ug/mL concentration shows positivity in nucleus and nucleoli.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human MKI67.
Immunogen
Recombinant protein corresponding to human MKI67.
Sequence
PAKVEDAADSATKPENLSSKTRGSIPTDVEVLPTETEIHNEPFLTLWLTQVERKIQKDSLSKPEKLGTTAGQMCSGLPGLSSVDINNFGDSINESEGIPLKRRRVSFGGHLRPELFDENLPPNTPLKRGEAPTKRKSLVMHTPPVLKKIIK
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:200-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human lymph node with MKI67 polyclonal antibody (Cat # PAB29347) shows strong nuclear positivity in a fraction of cells.Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with MKI67 polyclonal antibody (Cat # PAB29347) at 1-4 ug/mL concentration shows positivity in nucleus and nucleoli. -
Gene Info — MKI67
Entrez GeneID
4288Gene Name
MKI67
Gene Alias
KIA, Ki-67
Gene Description
antigen identified by monoclonal antibody Ki-67
Omim ID
176741Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq
Other Designations
OTTHUMP00000020739|proliferation-related Ki-67 antigen
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Interactome
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Disease
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