RPL30 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human RPL30.
Immunogen
Recombinant protein corresponding to amino acids of human RPL30.
Sequence
INSRLQLVMKSGKYVLGYKQTLKMIRQGKAKLVILANNCPALRKSEIEYYAMLAKTGVHHYSGNNIELGTACGKYYRVCTLAIIDPGDSDIIRSMPEQTGEK
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:200-1:500)
Western Blot (1:100-1:250)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Human cell line RT-4; Lane 2: Human cell line U-251MG sp with RPL30 polyclonal antibody (Cat# PAB29336) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebellum with RPL30 polyclonal antibody (Cat# PAB29336) shows cytoplasmic positivity in purkinje cells and cells of molecular layer at 1:200-1:500 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with RPL30 polyclonal antibody (Cat# PAB29336) under 1-4 ug/mL working concentration shows positivity in cytoplasm. -
Gene Info — RPL30
Entrez GeneID
6156Protein Accession#
P62888Gene Name
RPL30
Gene Alias
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Gene Description
ribosomal protein L30
Omim ID
180467Gene Ontology
HyperlinkGene Summary
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30E family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the U72 small nucleolar RNA gene, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq
Other Designations
60S ribosomal protein L30
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Interactome
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Pathway
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