Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with BIRC3 polyclonal antibody (Cat# PAB29332) at 1:100-1:250 dilution.
Immunohistochemical staining of human tonsil with BIRC3 polyclonal antibody (Cat# PAB29332) shows nuclear positivity in reaction center cells at 1:50-1:200 dilution.
Immunofluorescent staining of human cell line U-251 MG with BIRC3 polyclonal antibody (Cat# PAB29332) under 1-4 ug/mL working concentration shows positivity in cytoplasm and nucleus but excluded from the nucleoli.
The protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. The encoded protein inhibits apoptosis induced by serum deprivation but does not affect apoptosis resulting from exposure to menadione, a potent inducer of free radicals. The amino acid sequence predicts three baculovirus IAP repeat domains and a ring finger domain. Transcript variants encoding the same isoform have been identified. [provided by RefSeq
TNFR2-TRAF signaling complex protein,apoptosis inhibitor 2,baculoviral IAP repeat-containing protein 3,inhibitor of apoptosis protein 1,mammalian IAP homolog C