LEF1 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate); Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with LEF1 polyclonal antibody (Cat# PAB29327) at 1:100-1:250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human lymph node with LEF1 polyclonal antibody (Cat# PAB29327) shows strong nuclear positivity in non-germinal center cells at 1:50-1:200 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with LEF1 polyclonal antibody (Cat# PAB29327) under 1-4 ug/mL working concentration shows positivity in nucleus but excluded from the nucleoli.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human LEF1.
Immunogen
Recombinant protein corresponding to amino acids of human LEF1.
Sequence
SDVNSKQGMSRHPPAPDIPTFYPLSPGGVGQITPPLGWQGQPVYPITGGFRQPYPSSLSVDTSMSRFSHHMIPGPPGPHTTGIPHPAIVTPQVKQEHPHTDSDLMHVKPQHEQRKEQEPKRPHIKKPLNAFMLY
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:100-1:250)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate); Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with LEF1 polyclonal antibody (Cat# PAB29327) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human lymph node with LEF1 polyclonal antibody (Cat# PAB29327) shows strong nuclear positivity in non-germinal center cells at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with LEF1 polyclonal antibody (Cat# PAB29327) under 1-4 ug/mL working concentration shows positivity in nucleus but excluded from the nucleoli. -
Gene Info — LEF1
Entrez GeneID
51176Protein Accession#
Q9UJU2Gene Name
LEF1
Gene Alias
DKFZp586H0919, TCF1ALPHA
Gene Description
lymphoid enhancer-binding factor 1
Omim ID
153245Gene Ontology
HyperlinkGene Summary
LEF1 is a nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905) (Waterman et al., 1991 [PubMed 2010090]; van Genderen et al., 1994 [PubMed 7958926]).[supplied by OMIM
Other Designations
lymphoid enhancer binding factor-1
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Interactome
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Pathway
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Disease
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