FARSA polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human FARSA.
Immunogen
Recombinant protein corresponding to amino acids of human FARSA.
Sequence
EVIEAELRSTKHWELTAEGEEIAREGSHEARVFRSIPPEGLAQSELMRLPSGKVGFSKAMSNKWIRVDKSAADGPRVFRVVDSMEDEVQRRLQLVRGGQAEKLGEKERSELRKRKLLAEVTLKTYWVSKG
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:1000-1:2500)
Western Blot (1:100-1:250)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with FARSA polyclonal antibody (Cat# PAB29320) at 1:100-1:250 dilution.Western Blot
Western blot analysis of Lane 1: Human cell line RT-4; Lane 2: Human cell line U-251MG sp; Lane 3: Human plasma (IgG/HSA depleted); Lane 4: Human liver tissue; Lane 5: Human tonsil tissue with FARSA polyclonal antibody (Cat# PAB29320) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human duodenum with FARSA polyclonal antibody (Cat# PAB29320) shows strong cytoplasmic positivity in glandular cells at 1:1000-1:2500 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with FARSA polyclonal antibody (Cat# PAB29320) under 1-4 ug/mL working concentration shows positivity in cytoplasm. -
Gene Info — FARSA
Entrez GeneID
2193Gene Name
FARSA
Gene Alias
CML33, FARSL, FARSLA, FRSA, PheHA
Gene Description
phenylalanyl-tRNA synthetase, alpha subunit
Omim ID
602918Gene Ontology
HyperlinkGene Summary
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. This gene encodes a product which is similar to the catalytic subunit of prokaryotic and Saccharomyces cerevisiae phenylalanyl-tRNA synthetases (PheRS). This gene product has been shown to be expressed in a tumor-selective and cell cycle stage- and differentiation-dependent manner, the first member of the tRNA synthetase gene family shown to exhibit this type of regulated expression [provided by RefSeq
Other Designations
phenylalanine tRNA ligase 1, alpha, cytoplasmic|phenylalanine-tRNA synthetase alpha-subunit|phenylalanine-tRNA synthetase-like, alpha subunit|phenylalanyl-tRNA synthetase-like, alpha subunit
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Interactome
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Pathway
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