MLLT3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human MLLT3.
Immunogen
Recombinant protein corresponding to amino acids of human MLLT3.
Sequence
SSKSDSEQPSPASSSSSSSSSFTPSQTRQQGPLRSIMKDLHSDDNEEESDEVEDNDNDSEMERPVNRGGSRSRRVSLSDGSDSESSSASSPLHHEPPPPLLKTNNNQILEVKSPIKQSKSDKQIKNGECDKAYLDELVELHRRLMTLR
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:100-1:250)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate); Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with MLLT3 polyclonal antibody (Cat# PAB29315) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebellum with MLLT3 polyclonal antibody (Cat# PAB29315) shows strong nuclear positivity in purkinje cells at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with MLLT3 polyclonal antibody (Cat# PAB29315) under 1-4 ug/mL working concentration shows positivity in cytoplasm and nucleus but excluded from the nucleoli. -
Gene Info — MLLT3
Entrez GeneID
4300Protein Accession#
B7Z755Gene Name
MLLT3
Gene Alias
AF9, FLJ2035, YEATS3
Gene Description
myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3
Omim ID
159558Gene Ontology
HyperlinkGene Summary
Drosophila); translocated to
Other Designations
OTTHUMP00000021127|OTTHUMP00000045120|myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocated to, 3
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Interactome
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Disease
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