CD276 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human CD276.
Immunogen
Recombinant protein corresponding to amino acids 30-130 of human CD276.
Sequence
EVQVPEDPVVALVGTDATLCCSFSPEPGFSLAQLNLIWQLTDTKQLVHSFAEGQDQGSAYANRTALFPDLLAQGNASLRLQRVRVADEGSFTCFVSIRDFG
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (1:50-1:200)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with CD276 polyclonal antibody (Cat# PAB29313) at 1:100 - 1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human prostate tissue with CD276 polyclonal antibody (Cat# PAB29313) at 1:50 - 1:200 dilution.Immunofluorescence
Immunofluorescence staining of A-431 cells with CD276 polyclonal antibody (Cat# PAB29313) under 1-4 ug/mL working concentration. -
Gene Info — CD276
Entrez GeneID
80381Protein Accession#
Q5ZPR3Gene Name
CD276
Gene Alias
B7-H3, B7H3
Gene Description
CD276 molecule
Omim ID
605715Gene Ontology
HyperlinkGene Summary
Costimulatory B7 molecules (e.g., B7-1, or CD80; MIM 112203) signal through CD28 (MIM 186760) family molecules such as CD28, CTLA4 (MIM 123890), and ICOS (MIM 604558).[supplied by OMIM
Other Designations
B7 homolog 3|CD276 antigen
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Interactome
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Pathway
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