ENG polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: RT-4 cell lysate, Lane 2: U-251 MG sp cell lysate with ENG polyclonal antibody (Cat# PAB29312) at 1:100 - 1:250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human liver tissue with ENG polyclonal antibody (Cat# PAB29312) at 1:200 - 1:500 dilution.
Immunofluorescence
Immunofluorescence staining of U-251 MG cells with ENG polyclonal antibody (Cat# PAB29312) under 1-4 ug/mL working concentration.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human ENG.
Immunogen
Recombinant protein corresponding to amino acids 312-438 of human ENG.
Sequence
ASFVELPLASIVSLHASSCGGRLQTSPAPIQTTPPKDTCSPELLMSLIQTKCADDAMTLVLKKELVAHLKCTITGLTFWDPSCEAEDRGDKFVLRSAYSSCGMQVSASMISNEAVVNILSSSSPQRK
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (1:200-1:500)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: RT-4 cell lysate, Lane 2: U-251 MG sp cell lysate with ENG polyclonal antibody (Cat# PAB29312) at 1:100 - 1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human liver tissue with ENG polyclonal antibody (Cat# PAB29312) at 1:200 - 1:500 dilution.Immunofluorescence
Immunofluorescence staining of U-251 MG cells with ENG polyclonal antibody (Cat# PAB29312) under 1-4 ug/mL working concentration. -
Gene Info — ENG
Entrez GeneID
2022Protein Accession#
P17813Gene Name
ENG
Gene Alias
CD105, END, FLJ41744, HHT1, ORW, ORW1
Gene Description
endoglin
Gene Ontology
HyperlinkGene Summary
This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds TGFB1 and TGFB3 with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
CD105 antigen|OTTHUMP00000022221|Osler-Rendu-Weber syndrome 1
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Interactome
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Disease
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