EPHA3/EPHA4/EPHA5 (phospho Y779/Y833) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human EPHA3/EPHA4/EPHA5.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y779/Y833 of human EPHA3/EPHA4/EPHA5.
Host
Rabbit
Theoretical MW (kDa)
110
Reactivity
Human, Mouse
Specificity
EPHA3/EPHA4/EPHA5 (phospho Y779/Y833) polyclonal antibody detects endogenous levels of human EPHA3/EPHA4/EPHA5 only when phosphorylated at tyrosine 779/833.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunohistochemistry (1:50~1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human brain tissue with EPHA3/EPHA4/EPHA5 (phospho Y779/Y833) polyclonal antibody (Cat# PAB29297) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — EPHA3
Entrez GeneID
2042Protein Accession#
P29320;P54764;P54756Gene Name
EPHA3
Gene Alias
ETK, ETK1, HEK, HEK4, TYRO4
Gene Description
EPH receptor A3
Omim ID
179611Gene Ontology
HyperlinkGene Summary
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq
Other Designations
TYRO4 protein tyrosine kinase|eph-like tyrosine kinase 1|ephrin receptor EphA3|human embryo kinase 1
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Gene Info — EPHA4
Entrez GeneID
2043Protein Accession#
P29320;P54764;P54756Gene Name
EPHA4
Gene Alias
HEK8, SEK, TYRO1
Gene Description
EPH receptor A4
Omim ID
602188Gene Ontology
HyperlinkGene Summary
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq
Other Designations
OTTHUMP00000164185|TYRO1 protein tyrosine kinase|ephrin receptor EphA4|ephrin type-A receptor 4|receptor protein-tyrosine kinase HEK8|tyrosine-protein kinase receptor SEK
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Gene Info — EPHA5
Entrez GeneID
2044Protein Accession#
P29320;P54764;P54756Gene Name
EPHA5
Gene Alias
CEK7, EHK1, HEK7, TYRO4
Gene Description
EPH receptor A5
Omim ID
600004Gene Ontology
HyperlinkGene Summary
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Two transcript variants encoding different isoforms have been found for this gene.
Other Designations
Eph homology kinase-1|ephrin receptor EphA5|ephrin type-A receptor 5|receptor protein-tyrosine kinase HEK7|tyrosine-protein kinase receptor EHK-1
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Interactome
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Pathway
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Disease
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