CSNK1A1 (phospho Y294) polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human CSNK1A1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y294 of human CSNK1A1.
Host
Rabbit
Theoretical MW (kDa)
37
Reactivity
Human, Mouse, Rat
Specificity
CSNK1A1 (phospho Y294) polyclonal antibody detects endogenous levels of human CSNK1A1 only when phosphorylated at tyrosine 294.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunohistochemistry (1:50~1:100)
Western Blot (1:500~1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: heat shock treated HT-29 cells, Lane 2: antigen-specific peptide treated HT-29 cells with CSNK1A1 (phospho Y294) polyclonal antibody (Cat# PAB29296) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human brain tissue with CSNK1A1 (phospho Y294) polyclonal antibody (Cat# PAB29296) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — CSNK1A1
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Interactome
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Pathway
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Disease
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