STMN1 (phospho S63) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human STMN1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S63 of human STMN1.
Host
Rabbit
Theoretical MW (kDa)
22
Reactivity
Human, Mouse
Specificity
STMN1 (phospho S63) polyclonal antibody detects endogenous levels of human STMN1 only when phosphorylated at serine 63.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunohistochemistry (1:50~1:100)
Western Blot (1:500~1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: nocodazole treated COS-7 cells, Lane 2: antigen-specific peptide treated COS-7 cells with STMN1 (phospho S63) polyclonal antibody (Cat# PAB29293) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer tissue with STMN1 (phospho S63) polyclonal antibody (Cat# PAB29293) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — STMN1
Entrez GeneID
3925Protein Accession#
P16949Gene Name
STMN1
Gene Alias
LAP18, Lag, OP18, PP17, PP19, PR22, SMN
Gene Description
stathmin 1/oncoprotein 18
Omim ID
151442Gene Ontology
HyperlinkGene Summary
This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000008527|OTTHUMP00000008528|OTTHUMP00000008530|leukemia-associated phosphoprotein p18|metablastin|oncoprotein 18|phosphoprotein 19|prosolin|stathmin 1
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