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MUC1 (phospho Y1229) polyclonal antibody

  • Catalog # : PAB29286
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human MUC1.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y1229 of human MUC1.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 170
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • MUC1 (phospho Y1229) polyclonal antibody detects endogenous levels of human MUC1 only when phosphorylated at tyrosine 1229.
  • Form:
  • Liquid
  • Purification:
  • Affinity Chromatography
  • Recommend Usage:
  • Immunofluorescence (1:100~1:200)
    Immunohistochemistry (1:50~1:100)
    Western Blot (1:500~1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: PMA treated HepG2 cells, Lane 2: antigen-specific peptide treated HepG2 cells with MUC1 (phospho Y1229) polyclonal antibody (Cat# PAB29286) at 1:500-1:1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer tissue with MUC1 (phospho Y1229) polyclonal antibody (Cat# PAB29286) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of methanol-fixed HepG2 cells with MUC1 (phospho Y1229) polyclonal antibody (Cat# PAB29286) without blocking peptide (A) or preincubated with blocking peptide (B) at 1:100-1:200 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 4582
  • Gene Name:
  • MUC1
  • Gene Alias:
  • CD227,EMA,H23AG,MAM6,PEM,PEMT,PUM
  • Gene Description:
  • mucin 1, cell surface associated
  • Gene Summary:
  • This gene is a member of the mucin family and encodes a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length nature of only some has been determined. [provided by RefSeq
  • Other Designations:
  • DF3 antigen,H23 antigen,MUC-1/SEC,MUC-1/X,MUC1/ZD,OTTHUMP00000033787,OTTHUMP00000033790,OTTHUMP00000033795,breast carcinoma-associated antigen DF3,episialin,mucin 1,mucin 1, transmembrane,peanut-reactive urinary mucin,polymorphic epithelial mucin,tumor as
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