MUC1 (phospho Y1229) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: PMA treated HepG2 cells, Lane 2: antigen-specific peptide treated HepG2 cells with MUC1 (phospho Y1229) polyclonal antibody (Cat# PAB29286) at 1:500-1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer tissue with MUC1 (phospho Y1229) polyclonal antibody (Cat# PAB29286) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
Immunofluorescence
Immunofluorescence staining of methanol-fixed HepG2 cells with MUC1 (phospho Y1229) polyclonal antibody (Cat# PAB29286) without blocking peptide (A) or preincubated with blocking peptide (B) at 1:100-1:200 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human MUC1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y1229 of human MUC1.
Host
Rabbit
Theoretical MW (kDa)
170
Reactivity
Human, Mouse
Specificity
MUC1 (phospho Y1229) polyclonal antibody detects endogenous levels of human MUC1 only when phosphorylated at tyrosine 1229.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunofluorescence (1:100~1:200)
Immunohistochemistry (1:50~1:100)
Western Blot (1:500~1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: PMA treated HepG2 cells, Lane 2: antigen-specific peptide treated HepG2 cells with MUC1 (phospho Y1229) polyclonal antibody (Cat# PAB29286) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer tissue with MUC1 (phospho Y1229) polyclonal antibody (Cat# PAB29286) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.Immunofluorescence
Immunofluorescence staining of methanol-fixed HepG2 cells with MUC1 (phospho Y1229) polyclonal antibody (Cat# PAB29286) without blocking peptide (A) or preincubated with blocking peptide (B) at 1:100-1:200 dilution. -
Gene Info — MUC1
Entrez GeneID
4582Protein Accession#
P15941Gene Name
MUC1
Gene Alias
CD227, EMA, H23AG, MAM6, PEM, PEMT, PUM
Gene Description
mucin 1, cell surface associated
Omim ID
158340Gene Ontology
HyperlinkGene Summary
This gene is a member of the mucin family and encodes a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length nature of only some has been determined. [provided by RefSeq
Other Designations
DF3 antigen|H23 antigen|MUC-1/SEC|MUC-1/X|MUC1/ZD|OTTHUMP00000033787|OTTHUMP00000033790|OTTHUMP00000033795|breast carcinoma-associated antigen DF3|episialin|mucin 1|mucin 1, transmembrane|peanut-reactive urinary mucin|polymorphic epithelial mucin|tumor as
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Interactome
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Disease
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