NR4A1 (phospho S351) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human NR4A1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S351 of human NR4A1.
Host
Rabbit
Theoretical MW (kDa)
64
Reactivity
Human
Specificity
NR4A1 (phospho S351) polyclonal antibody detects endogenous levels of human NR4A1 only when phosphorylated at serine 351.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunohistochemistry (1:50~1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon cancer tissue with NR4A1 (phospho S351) polyclonal antibody (Cat# PAB29285) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — NR4A1
Entrez GeneID
3164Protein Accession#
P22736Gene Name
NR4A1
Gene Alias
GFRP1, HMR, MGC9485, N10, NAK-1, NGFIB, NP10, NUR77, TR3
Gene Description
nuclear receptor subfamily 4, group A, member 1
Omim ID
139139Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
TR3 orphan receptor|early response protein NAK1|growth factor-inducible nuclear protein N10|hormone receptor|orphan nuclear receptor HMR|steroid receptor TR3
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