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HNRNPD (phospho S83) polyclonal antibody

  • Catalog # : PAB29280
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human HNRNPD.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S83 of human HNRNPD.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 38
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • HNRNPD (phospho S83) polyclonal antibody detects endogenous levels of human HNRNPD only when phosphorylated at serine 83.
  • Form:
  • Liquid
  • Purification:
  • Affinity Chromatography
  • Recommend Usage:
  • Immunofluorescence (1:100~1:200)
    Immunohistochemistry (1:50~1:100)
    Western Blot (1:500~1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: antigen-specific peptide treated JK cells, Lane 2: JK cells with HNRNPD (phospho S83) polyclonal antibody (Cat# PAB29280) at 1:500-1:1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lung cancer tissue with HNRNPD (phospho S83) polyclonal antibody (Cat# PAB29280) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of methanol-fixed HeLa cells with HNRNPD (phospho S83) polyclonal antibody (Cat# PAB29280) without blocking peptide (A) or preincubated with blocking peptide (B) at 1:100-1:200 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 3184
  • Gene Name:
  • HNRNPD
  • Gene Alias:
  • AUF1,AUF1A,HNRPD,P37,hnRNPD0
  • Gene Description:
  • heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa)
  • Gene Summary:
  • This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants. [provided by RefSeq
  • Other Designations:
  • ARE-binding protein AUFI, type A,AU-rich element RNA-binding protein 1,heterogeneous nuclear ribonucleoprotein D,heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA-binding protein 1, 37kD)
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