HNRNPD (phospho S83) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: antigen-specific peptide treated JK cells, Lane 2: JK cells with HNRNPD (phospho S83) polyclonal antibody (Cat# PAB29280) at 1:500-1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lung cancer tissue with HNRNPD (phospho S83) polyclonal antibody (Cat# PAB29280) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells with HNRNPD (phospho S83) polyclonal antibody (Cat# PAB29280) without blocking peptide (A) or preincubated with blocking peptide (B) at 1:100-1:200 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human HNRNPD.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S83 of human HNRNPD.
Host
Rabbit
Theoretical MW (kDa)
38
Reactivity
Human, Mouse, Rat
Specificity
HNRNPD (phospho S83) polyclonal antibody detects endogenous levels of human HNRNPD only when phosphorylated at serine 83.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunofluorescence (1:100~1:200)
Immunohistochemistry (1:50~1:100)
Western Blot (1:500~1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: antigen-specific peptide treated JK cells, Lane 2: JK cells with HNRNPD (phospho S83) polyclonal antibody (Cat# PAB29280) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lung cancer tissue with HNRNPD (phospho S83) polyclonal antibody (Cat# PAB29280) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells with HNRNPD (phospho S83) polyclonal antibody (Cat# PAB29280) without blocking peptide (A) or preincubated with blocking peptide (B) at 1:100-1:200 dilution. -
Gene Info — HNRNPD
Entrez GeneID
3184Protein Accession#
Q14103Gene Name
HNRNPD
Gene Alias
AUF1, AUF1A, HNRPD, P37, hnRNPD0
Gene Description
heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa)
Omim ID
601324Gene Ontology
HyperlinkGene Summary
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants. [provided by RefSeq
Other Designations
ARE-binding protein AUFI, type A|AU-rich element RNA-binding protein 1|heterogeneous nuclear ribonucleoprotein D|heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA-binding protein 1, 37kD)
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