HRH1 (phospho S398) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human HRH1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S398 of human HRH1.
Host
Rabbit
Theoretical MW (kDa)
55
Reactivity
Human, Mouse
Specificity
HRH1 (phospho S398) polyclonal antibody detects endogenous levels of human HRH1 only when phosphorylated at serine 398.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunofluorescence (1:100~1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunofluorescence
Immunofluorescence staining of methanol-fixed HUVEC cells with HRH1 (phospho S398) polyclonal antibody (Cat# PAB29279) without blocking peptide (A) or preincubated with blocking peptide (B) at 1:100-1:200 dilution. -
Gene Info — HRH1
Entrez GeneID
3269Protein Accession#
P35367Gene Name
HRH1
Gene Alias
H1-R, hisH1
Gene Description
histamine receptor H1
Omim ID
600167Gene Ontology
HyperlinkGene Summary
Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene was thought to be intronless until recently. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000160133|histamine H(1) receptor|histamine receptor, subclass H1
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