EPHA2/EPHA3/EPHA4 (phospho Y588/Y596) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cells, Lane 2: antigen-specific peptide treated HepG2 cells with EPHA2/EPHA3/EPHA4 (phospho Y588/Y596) polyclonal antibody (Cat# PAB29278) at 1:500-1:1000 dilution.
Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells with EPHA2/EPHA3/EPHA4 (phospho Y588/Y596) polyclonal antibody (Cat# PAB29278) without blocking peptide (A) or preincubated with blocking peptide (B) at 1:100-1:200 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human EPHA2/EPHA3/EPHA4.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y588/Y596 of human EPHA2/EPHA3/EPHA4.
Host
Rabbit
Theoretical MW (kDa)
130
Reactivity
Human, Mouse
Specificity
EPHA2/EPHA3/EPHA4 (phospho Y588/Y596) polyclonal antibody detects endogenous levels of human EPHA2/EPHA3/EPHA4 only when phosphorylated at tyrosine 588/596.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunofluorescence (1:100~1:200)
Western Blot (1:500~1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cells, Lane 2: antigen-specific peptide treated HepG2 cells with EPHA2/EPHA3/EPHA4 (phospho Y588/Y596) polyclonal antibody (Cat# PAB29278) at 1:500-1:1000 dilution.Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells with EPHA2/EPHA3/EPHA4 (phospho Y588/Y596) polyclonal antibody (Cat# PAB29278) without blocking peptide (A) or preincubated with blocking peptide (B) at 1:100-1:200 dilution. -
Gene Info — EPHA2
Entrez GeneID
1969Protein Accession#
P29317;P29320;P54764Gene Name
EPHA2
Gene Alias
ECK
Gene Description
EPH receptor A2
Omim ID
176946Gene Ontology
HyperlinkGene Summary
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. [provided by RefSeq
Other Designations
ephrin receptor EphA2|epithelial cell receptor protein tyrosine kinase|protein tyrosine kinase|receptor protein tyrosine kinase regulated by p53 and E2F-1|soluble EPHA2 variant 1
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Gene Info — EPHA3
Entrez GeneID
2042Protein Accession#
P29317;P29320;P54764Gene Name
EPHA3
Gene Alias
ETK, ETK1, HEK, HEK4, TYRO4
Gene Description
EPH receptor A3
Omim ID
179611Gene Ontology
HyperlinkGene Summary
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq
Other Designations
TYRO4 protein tyrosine kinase|eph-like tyrosine kinase 1|ephrin receptor EphA3|human embryo kinase 1
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Gene Info — EPHA4
Entrez GeneID
2043Protein Accession#
P29317;P29320;P54764Gene Name
EPHA4
Gene Alias
HEK8, SEK, TYRO1
Gene Description
EPH receptor A4
Omim ID
602188Gene Ontology
HyperlinkGene Summary
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq
Other Designations
OTTHUMP00000164185|TYRO1 protein tyrosine kinase|ephrin receptor EphA4|ephrin type-A receptor 4|receptor protein-tyrosine kinase HEK8|tyrosine-protein kinase receptor SEK
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Interactome
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Pathway
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Disease
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