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EPHA2/EPHA3/EPHA4 (phospho Y588/Y596) polyclonal antibody

  • Catalog # : PAB29278
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human EPHA2/EPHA3/EPHA4.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y588/Y596 of human EPHA2/EPHA3/EPHA4.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 130
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • EPHA2/EPHA3/EPHA4 (phospho Y588/Y596) polyclonal antibody detects endogenous levels of human EPHA2/EPHA3/EPHA4 only when phosphorylated at tyrosine 588/596.
  • Form:
  • Liquid
  • Purification:
  • Affinity Chromatography
  • Recommend Usage:
  • Immunofluorescence (1:100~1:200)
    Western Blot (1:500~1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: HepG2 cells, Lane 2: antigen-specific peptide treated HepG2 cells with EPHA2/EPHA3/EPHA4 (phospho Y588/Y596) polyclonal antibody (Cat# PAB29278) at 1:500-1:1000 dilution.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of methanol-fixed HeLa cells with EPHA2/EPHA3/EPHA4 (phospho Y588/Y596) polyclonal antibody (Cat# PAB29278) without blocking peptide (A) or preincubated with blocking peptide (B) at 1:100-1:200 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 1969
  • Protein Accession#:
  • P29317;P29320;P54764
  • Gene Name:
  • EPHA2
  • Gene Alias:
  • ECK
  • Gene Description:
  • EPH receptor A2
  • Gene Summary:
  • This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. [provided by RefSeq
  • Other Designations:
  • ephrin receptor EphA2,epithelial cell receptor protein tyrosine kinase,protein tyrosine kinase,receptor protein tyrosine kinase regulated by p53 and E2F-1,soluble EPHA2 variant 1
  • Gene Information
  • Entrez GeneID:
  • 2042
  • Protein Accession#:
  • P29317;P29320;P54764
  • Gene Name:
  • EPHA3
  • Gene Alias:
  • ETK,ETK1,HEK,HEK4,TYRO4
  • Gene Description:
  • EPH receptor A3
  • Gene Summary:
  • This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq
  • Other Designations:
  • TYRO4 protein tyrosine kinase,eph-like tyrosine kinase 1,ephrin receptor EphA3,human embryo kinase 1
  • Gene Information
  • Entrez GeneID:
  • 2043
  • Protein Accession#:
  • P29317;P29320;P54764
  • Gene Name:
  • EPHA4
  • Gene Alias:
  • HEK8,SEK,TYRO1
  • Gene Description:
  • EPH receptor A4
  • Gene Summary:
  • This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000164185,TYRO1 protein tyrosine kinase,ephrin receptor EphA4,ephrin type-A receptor 4,receptor protein-tyrosine kinase HEK8,tyrosine-protein kinase receptor SEK
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