BAD (phospho S91/S128) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: TNF-a treated COS-7 cells, Lane 2: antigen-specific peptide treated COS-7 cells with BAD (phospho S91/S128) polyclonal antibody (Cat# PAB29276) at 1:500-1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer tissue with BAD (phospho S91/S128) polyclonal antibody (Cat# PAB29276) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human BAD.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S91 of human BAD.
Host
Rabbit
Theoretical MW (kDa)
22
Reactivity
Human, Mouse
Specificity
BAD (phospho S91/S128) polyclonal antibody detects endogenous levels of human BAD only when phosphorylated at serine 91/serine 128.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunohistochemistry (1:50~1:100)
Western Blot (1:500~1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: TNF-a treated COS-7 cells, Lane 2: antigen-specific peptide treated COS-7 cells with BAD (phospho S91/S128) polyclonal antibody (Cat# PAB29276) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer tissue with BAD (phospho S91/S128) polyclonal antibody (Cat# PAB29276) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — BAD
Entrez GeneID
572Protein Accession#
Q92934Gene Name
BAD
Gene Alias
BBC2, BCL2L8
Gene Description
BCL2-associated agonist of cell death
Omim ID
603167Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq
Other Designations
BCL-X/BCL-2 binding protein|BCL2-antagonist of cell death protein|BCL2-binding component 6|BCL2-binding protein
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Interactome
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Pathway
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Disease
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