CHEK2 (phospho T383) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: UV treated COS-7 cells, Lane 2: antigen-specific peptide treated COS-7 cells with CHEK2 (phospho T383) polyclonal antibody (Cat# PAB29275) at 1:500-1:1000 dilution.
Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells with CHEK2 (phospho T383) polyclonal antibody (Cat# PAB29275) without blocking peptide (A) or preincubated with blocking peptide (B) at 1:100-1:200 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human CHEK2.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T383 of human CHEK2.
Host
Rabbit
Theoretical MW (kDa)
60
Reactivity
Human, Mouse, Rat
Specificity
CHEK2 (phospho T383) polyclonal antibody detects endogenous levels of human CHEK2 only when phosphorylated at threonine 383.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunofluorescence (1:100~1:200)
Western Blot (1:500~1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: UV treated COS-7 cells, Lane 2: antigen-specific peptide treated COS-7 cells with CHEK2 (phospho T383) polyclonal antibody (Cat# PAB29275) at 1:500-1:1000 dilution.Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells with CHEK2 (phospho T383) polyclonal antibody (Cat# PAB29275) without blocking peptide (A) or preincubated with blocking peptide (B) at 1:100-1:200 dilution. -
Gene Info — CHEK2
Entrez GeneID
11200Protein Accession#
O96017Gene Name
CHEK2
Gene Alias
CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53
Gene Description
CHK2 checkpoint homolog (S. pombe)
Gene Ontology
HyperlinkGene Summary
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000028871|checkpoint-like protein CHK2|protein kinase CHK2|serine/threonine-protein kinase CHK2
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Disease
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