BLM (phospho T99) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human BLM.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T99 of human BLM.
Host
Rabbit
Theoretical MW (kDa)
159
Reactivity
Human, Mouse, Rat
Specificity
BLM (phospho T99) polyclonal antibody detects endogenous levels of human BLM only when phosphorylated at threonine 99.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunohistochemistry (1:50~1:100)
Western Blot (1:500~1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cells, Lane 2: antigen-specific peptide treated HepG2 cells with BLM (phospho T99) polyclonal antibody (Cat# PAB29273) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human heart tissue with BLM (phospho T99) polyclonal antibody (Cat# PAB29273) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — BLM
Entrez GeneID
641Protein Accession#
P54132Gene Name
BLM
Gene Alias
BS, MGC126616, MGC131618, MGC131620, RECQ2, RECQL2, RECQL3
Gene Description
Bloom syndrome
Gene Ontology
HyperlinkGene Summary
The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq
Other Designations
Bloom syndrome protein
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Interactome
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Pathway
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Disease
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