PEA15 (phospho S104) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: TNF treated COS-7 cells, Lane 2: antigen-specific peptide treated COS-7 cells with PEA15 (phospho S104) polyclonal antibody (Cat# PAB29270) at 1:500-1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human heart tissue with PEA15 (phospho S104) polyclonal antibody (Cat# PAB29270) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human PEA15.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S104 of human PEA15.
Host
Rabbit
Theoretical MW (kDa)
19
Reactivity
Human, Mouse, Rat
Specificity
PEA15 (phospho S104) polyclonal antibody detects endogenous levels of human PEA15 only when phosphorylated at serine 104.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunohistochemistry (1:50~1:100)
Western Blot (1:500~1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: TNF treated COS-7 cells, Lane 2: antigen-specific peptide treated COS-7 cells with PEA15 (phospho S104) polyclonal antibody (Cat# PAB29270) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human heart tissue with PEA15 (phospho S104) polyclonal antibody (Cat# PAB29270) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — PEA15
Entrez GeneID
8682Protein Accession#
Q15121Gene Name
PEA15
Gene Alias
HMAT1, HUMMAT1H, MAT1, MAT1H, PEA-15, PED
Gene Description
phosphoprotein enriched in astrocytes 15
Omim ID
603434Gene Ontology
HyperlinkGene Summary
PEA15 is a death effector domain (DED)-containing protein predominantly expressed in the central nervous system, particularly in astrocytes.[supplied by OMIM
Other Designations
OTTHUMP00000029886|Phosphoprotein enriched in astrocytes, 15kD|homolog of mouse MAT-1 oncogene
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Interactome
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Disease
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