CHEK1 (phospho S296) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: UV treated HUVEC cells, Lane 2: antigen-specific peptide treated HUVEC cells with CHEK1 (phospho S296) polyclonal antibody (Cat# PAB29268) at 1:500-1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human brain tissue with CHEK1 (phospho S296) polyclonal antibody (Cat# PAB29268) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human CHEK1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S296 of human CHEK1.
Host
Rabbit
Theoretical MW (kDa)
45
Reactivity
Human, Mouse, Rat
Specificity
CHEK1 (phospho S296) polyclonal antibody detects endogenous levels of human CHEK1 only when phosphorylated at serine 296.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunohistochemistry (1:50~1:100)
Western Blot (1:500~1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: UV treated HUVEC cells, Lane 2: antigen-specific peptide treated HUVEC cells with CHEK1 (phospho S296) polyclonal antibody (Cat# PAB29268) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human brain tissue with CHEK1 (phospho S296) polyclonal antibody (Cat# PAB29268) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — CHEK1
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Interactome
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Pathway
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Disease
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