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Last updated: 2017/5/28

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CHEK1 (phospho S296) polyclonal antibody

  • Catalog # : PAB29268
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human CHEK1.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S296 of human CHEK1.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 45
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • CHEK1 (phospho S296) polyclonal antibody detects endogenous levels of human CHEK1 only when phosphorylated at serine 296.
  • Form:
  • Liquid
  • Purification:
  • Affinity Chromatography
  • Recommend Usage:
  • Immunohistochemistry (1:50~1:100)
    Western Blot (1:500~1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: UV treated HUVEC cells, Lane 2: antigen-specific peptide treated HUVEC cells with CHEK1 (phospho S296) polyclonal antibody (Cat# PAB29268) at 1:500-1:1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human brain tissue with CHEK1 (phospho S296) polyclonal antibody (Cat# PAB29268) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 1111
  • Gene Name:
  • CHEK1
  • Gene Alias:
  • CHK1
  • Gene Description:
  • CHK1 checkpoint homolog (S. pombe)
  • Other Designations:
  • CHK1 checkpoint homolog,Checkpoint, S. pombe, homolog of, 1
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