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TNK2 (phospho Y284) polyclonal antibody

  • Catalog # : PAB29267
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human TNK2.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y284 of human TNK2.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 120
  • Reactivity:
  • Human
  • Specificity:
  • TNK2 (phospho Y284) polyclonal antibody detects endogenous levels of human TNK2 only when phosphorylated at tyrosine 284.
  • Form:
  • Liquid
  • Purification:
  • Affinity Chromatography
  • Recommend Usage:
  • Immunofluorescence (1:100~1:200)
    Immunohistochemistry (1:50~1:100)
    Western Blot (1:500~1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: EGF treated HepG2 cells, Lane 2: antigen-specific peptide treated HepG2 cells with TNK2 (phospho Y284) polyclonal antibody (Cat# PAB29267) at 1:500-1:1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer tissue with TNK2 (phospho Y284) polyclonal antibody (Cat# PAB29267) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of methanol-fixed A-549 cells with TNK2 (phospho Y284) polyclonal antibody (Cat# PAB29267) without blocking peptide (A) or preincubated with blocking peptide (B) at 1:100-1:200 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Gene Name:
  • TNK2
  • Gene Alias:
  • ACK,ACK1,FLJ44758,FLJ45547,p21cdc42Hs
  • Gene Description:
  • tyrosine kinase, non-receptor, 2
  • Gene Summary:
  • This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq
  • Other Designations:
  • activated Cdc42-associated kinase 1,activated p21cdc42Hs kinase
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