TNK2 (phospho Y284) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: EGF treated HepG2 cells, Lane 2: antigen-specific peptide treated HepG2 cells with TNK2 (phospho Y284) polyclonal antibody (Cat# PAB29267) at 1:500-1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer tissue with TNK2 (phospho Y284) polyclonal antibody (Cat# PAB29267) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
Immunofluorescence
Immunofluorescence staining of methanol-fixed A-549 cells with TNK2 (phospho Y284) polyclonal antibody (Cat# PAB29267) without blocking peptide (A) or preincubated with blocking peptide (B) at 1:100-1:200 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human TNK2.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y284 of human TNK2.
Host
Rabbit
Theoretical MW (kDa)
120
Reactivity
Human
Specificity
TNK2 (phospho Y284) polyclonal antibody detects endogenous levels of human TNK2 only when phosphorylated at tyrosine 284.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunofluorescence (1:100~1:200)
Immunohistochemistry (1:50~1:100)
Western Blot (1:500~1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: EGF treated HepG2 cells, Lane 2: antigen-specific peptide treated HepG2 cells with TNK2 (phospho Y284) polyclonal antibody (Cat# PAB29267) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer tissue with TNK2 (phospho Y284) polyclonal antibody (Cat# PAB29267) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.Immunofluorescence
Immunofluorescence staining of methanol-fixed A-549 cells with TNK2 (phospho Y284) polyclonal antibody (Cat# PAB29267) without blocking peptide (A) or preincubated with blocking peptide (B) at 1:100-1:200 dilution. -
Gene Info — TNK2
Entrez GeneID
10188Protein Accession#
Q07912Gene Name
TNK2
Gene Alias
ACK, ACK1, FLJ44758, FLJ45547, p21cdc42Hs
Gene Description
tyrosine kinase, non-receptor, 2
Omim ID
606994Gene Ontology
HyperlinkGene Summary
This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq
Other Designations
activated Cdc42-associated kinase 1|activated p21cdc42Hs kinase
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