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PRKACA/PRKACB (phospho T197) polyclonal antibody

  • Catalog # : PAB29264
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human PRKACA/PRKACB.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T197 of human PRKACA/PRKACB.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 40
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • PRKACA/PRKACB (phospho T197) polyclonal antibody detects endogenous levels of human PRKACA/PRKACB only when phosphorylated at threonine 197.
  • Form:
  • Liquid
  • Purification:
  • Affinity Chromatography
  • Recommend Usage:
  • Immunofluorescence (1:100~1:200)
    Immunohistochemistry (1:50~1:100)
    Western Blot (1:500~1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot analysis of Lane 1: mouse brain tissue, Lane 2: antigen-specific peptide treated mouse brain tissue with PRKACA/PRKACB (phospho T197) polyclonal antibody (Cat# PAB29264) at 1:500-1:1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon cancer tissue with PRKACA/PRKACB (phospho T197) polyclonal antibody (Cat# PAB29264) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of methanol-fixed A-549 cells with PRKACA/PRKACB (phospho T197) polyclonal antibody (Cat# PAB29264) without blocking peptide (A) or preincubated with blocking peptide (B) at 1:100-1:200 dilution.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 5566
  • Gene Name:
  • PRKACA
  • Gene Alias:
  • MGC102831,MGC48865,PKACA
  • Gene Description:
  • protein kinase, cAMP-dependent, catalytic, alpha
  • Gene Summary:
  • cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
  • Other Designations:
  • PKA C-alpha,cAMP-dependent protein kinase catalytic subunit alpha,cAMP-dependent protein kinase catalytic subunit alpha, isoform 1,protein kinase A catalytic subunit
  • Gene Information
  • Entrez GeneID:
  • 5567
  • Gene Name:
  • PRKACB
  • Gene Alias:
  • DKFZp781I2452,MGC41879,MGC9320,PKACB
  • Gene Description:
  • protein kinase, cAMP-dependent, catalytic, beta
  • Gene Summary:
  • cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Three alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000011663,OTTHUMP00000011664,OTTHUMP00000011666,PKA C-beta,cAMP-dependent protein kinase catalytic beta subunit isoform 4ab,cAMP-dependent protein kinase catalytic subunit beta,protein kinase A catalytic subunit beta
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