PRKACA/PRKACB (phospho T197) polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot analysis of Lane 1: mouse brain tissue, Lane 2: antigen-specific peptide treated mouse brain tissue with PRKACA/PRKACB (phospho T197) polyclonal antibody (Cat# PAB29264) at 1:500-1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon cancer tissue with PRKACA/PRKACB (phospho T197) polyclonal antibody (Cat# PAB29264) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
Immunofluorescence
Immunofluorescence staining of methanol-fixed A-549 cells with PRKACA/PRKACB (phospho T197) polyclonal antibody (Cat# PAB29264) without blocking peptide (A) or preincubated with blocking peptide (B) at 1:100-1:200 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human PRKACA/PRKACB.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T197 of human PRKACA/PRKACB.
Host
Rabbit
Theoretical MW (kDa)
40
Reactivity
Human, Mouse, Rat
Specificity
PRKACA/PRKACB (phospho T197) polyclonal antibody detects endogenous levels of human PRKACA/PRKACB only when phosphorylated at threonine 197.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunofluorescence (1:100~1:200)
Immunohistochemistry (1:50~1:100)
Western Blot (1:500~1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of Lane 1: mouse brain tissue, Lane 2: antigen-specific peptide treated mouse brain tissue with PRKACA/PRKACB (phospho T197) polyclonal antibody (Cat# PAB29264) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon cancer tissue with PRKACA/PRKACB (phospho T197) polyclonal antibody (Cat# PAB29264) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.Immunofluorescence
Immunofluorescence staining of methanol-fixed A-549 cells with PRKACA/PRKACB (phospho T197) polyclonal antibody (Cat# PAB29264) without blocking peptide (A) or preincubated with blocking peptide (B) at 1:100-1:200 dilution. -
Gene Info — PRKACA
Entrez GeneID
5566Protein Accession#
P17612Gene Name
PRKACA
Gene Alias
MGC102831, MGC48865, PKACA
Gene Description
protein kinase, cAMP-dependent, catalytic, alpha
Omim ID
601639Gene Ontology
HyperlinkGene Summary
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
PKA C-alpha|cAMP-dependent protein kinase catalytic subunit alpha|cAMP-dependent protein kinase catalytic subunit alpha, isoform 1|protein kinase A catalytic subunit
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Gene Info — PRKACB
Entrez GeneID
5567Protein Accession#
P17612Gene Name
PRKACB
Gene Alias
DKFZp781I2452, MGC41879, MGC9320, PKACB
Gene Description
protein kinase, cAMP-dependent, catalytic, beta
Omim ID
176892Gene Ontology
HyperlinkGene Summary
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Three alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
OTTHUMP00000011663|OTTHUMP00000011664|OTTHUMP00000011666|PKA C-beta|cAMP-dependent protein kinase catalytic beta subunit isoform 4ab|cAMP-dependent protein kinase catalytic subunit beta|protein kinase A catalytic subunit beta
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Interactome
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Pathway
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Disease
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