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ETS1 (phospho T38) polyclonal antibody

  • Catalog # : PAB29261
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human ETS1.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T38 of human ETS1.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 54
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • ETS1 (phospho T38) polyclonal antibody detects endogenous levels of human ETS1 only when phosphorylated at threonine 38.
  • Form:
  • Liquid
  • Purification:
  • Affinity Chromatography
  • Recommend Usage:
  • Immunofluorescence (1:100~1:200)
    Immunohistochemistry (1:50~1:100)
    Western Blot (1:500~1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: HepG2 cells, Lane 2: antigen-specific peptide treated HepG2 cells with ETS1 (phospho T38) polyclonal antibody (Cat# PAB29261) at 1:500-1:1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer tissue with ETS1 (phospho T38) polyclonal antibody (Cat# PAB29261) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence staining of methanol-fixed HeLa cells with ETS1 (phospho T38) polyclonal antibody (Cat# PAB29261) without blocking peptide (A) or preincubated with blocking peptide (B) at 1:100-1:200 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 2113
  • Gene Name:
  • ETS1
  • Gene Alias:
  • ETS-1,EWSR2,FLJ10768
  • Gene Description:
  • v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)
  • Gene Summary:
  • ETS transcriptions factors, such as ETS1, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (Dwyer et al., 2007 [PubMed 17986575]).[supplied by OMIM
  • Other Designations:
  • Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1,ets protein,v-ets avian erythroblastosis virus E2 oncogene homolog 1,v-ets avian erythroblastosis virus E26 oncogene homolog 1,v-ets erythroblastosis virus E26 oncogene homolog 1
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