ETS1 (phospho T38) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cells, Lane 2: antigen-specific peptide treated HepG2 cells with ETS1 (phospho T38) polyclonal antibody (Cat# PAB29261) at 1:500-1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer tissue with ETS1 (phospho T38) polyclonal antibody (Cat# PAB29261) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells with ETS1 (phospho T38) polyclonal antibody (Cat# PAB29261) without blocking peptide (A) or preincubated with blocking peptide (B) at 1:100-1:200 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human ETS1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T38 of human ETS1.
Host
Rabbit
Theoretical MW (kDa)
54
Reactivity
Human, Mouse, Rat
Specificity
ETS1 (phospho T38) polyclonal antibody detects endogenous levels of human ETS1 only when phosphorylated at threonine 38.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunofluorescence (1:100~1:200)
Immunohistochemistry (1:50~1:100)
Western Blot (1:500~1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cells, Lane 2: antigen-specific peptide treated HepG2 cells with ETS1 (phospho T38) polyclonal antibody (Cat# PAB29261) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer tissue with ETS1 (phospho T38) polyclonal antibody (Cat# PAB29261) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells with ETS1 (phospho T38) polyclonal antibody (Cat# PAB29261) without blocking peptide (A) or preincubated with blocking peptide (B) at 1:100-1:200 dilution. -
Gene Info — ETS1
Entrez GeneID
2113Protein Accession#
P27577Gene Name
ETS1
Gene Alias
ETS-1, EWSR2, FLJ10768
Gene Description
v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)
Omim ID
164720Gene Ontology
HyperlinkGene Summary
ETS transcriptions factors, such as ETS1, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (Dwyer et al., 2007 [PubMed 17986575]).[supplied by OMIM
Other Designations
Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1|ets protein|v-ets avian erythroblastosis virus E2 oncogene homolog 1|v-ets avian erythroblastosis virus E26 oncogene homolog 1|v-ets erythroblastosis virus E26 oncogene homolog 1
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Interactome
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Pathway
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Disease
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