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CEBPA (phospho S21) polyclonal antibody

  • Catalog # : PAB29256
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human CEBPA.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S21 of human CEBPA.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 45
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • CEBPA (phospho S21) polyclonal antibody detects endogenous levels of human CEBPA only when phosphorylated at serine 21.
  • Form:
  • Liquid
  • Purification:
  • Affinity Chromatography
  • Recommend Usage:
  • Immunohistochemistry (1:50~1:100)
    Western Blot (1:500~1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: EGF treated HepG2 cells, Lane 2: antigen-specific peptide treated HepG2 cells with CEBPA (phospho S21) polyclonal antibody (Cat# PAB29256) at 1:500-1:1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon cancer tissue with CEBPA (phospho S21) polyclonal antibody (Cat# PAB29256) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 1050
  • Gene Name:
  • CEBPA
  • Gene Alias:
  • C/EBP-alpha,CEBP
  • Gene Description:
  • CCAAT/enhancer binding protein (C/EBP), alpha
  • Gene Summary:
  • The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. [provided by RefSeq
  • Other Designations:
  • CCAAT/enhancer binding protein alpha
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