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CAMK2A/CAMK2B/CAMK2D (phospho T305) polyclonal antibody

  • Catalog # : PAB29254
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human CAMK2A/CAMK2B/CAMK2D.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T305 of human CAMK2A/CAMK2B/CAMK2D.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 54
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • CAMK2A/CAMK2B/CAMK2D (phospho T305) polyclonal antibody detects endogenous levels of human CAMK2A/CAMK2B/CAMK2D only when phosphorylated at threonine 305.
  • Form:
  • Liquid
  • Purification:
  • Affinity Chromatography
  • Recommend Usage:
  • Immunohistochemistry (1:50~1:100)
    Western Blot (1:500~1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: antigen-specific peptide treated 3T3 cells, Lane 2: 3T3 cells with CAMK2A/CAMK2B/CAMK2D (phospho T305) polyclonal antibody (Cat# PAB29254) at 1:500-1:1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human brain tissue with CAMK2A/CAMK2B/CAMK2D (phospho T305) polyclonal antibody (Cat# PAB29254) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 815
  • Gene Name:
  • CAMK2A
  • Gene Alias:
  • CAMKA,KIAA0968
  • Gene Description:
  • calcium/calmodulin-dependent protein kinase II alpha
  • Gene Summary:
  • The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
  • Other Designations:
  • CaM kinase II alpha subunit,CaM-kinase II alpha chain,CaMK-II alpha subunit,CaMKIINalpha,OTTHUMP00000165787,OTTHUMP00000165788,calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha,calcium/calmodulin-dependent protein kinase II alpha-B subunit
  • Gene Information
  • Entrez GeneID:
  • 816
  • Gene Name:
  • CAMK2B
  • Gene Alias:
  • CAM2,CAMK2,CAMKB,MGC29528
  • Gene Description:
  • calcium/calmodulin-dependent protein kinase II beta
  • Gene Summary:
  • The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Eight transcript variants encoding eight distinct isoforms have been identified for this gene. [provided by RefSeq
  • Other Designations:
  • CaM kinase II beta subunit,CaM-kinase II beta chain,CaMK-II beta subunit,OTTHUMP00000159355,OTTHUMP00000159356,calcium/calmodulin-dependent protein kinase (CaM kinase) II beta,calcium/calmodulin-dependent protein kinase type II beta chain,proline rich cal
  • Gene Information
  • Entrez GeneID:
  • 817
  • Gene Name:
  • CAMK2D
  • Gene Alias:
  • CAMKD,DKFZp686G23119,DKFZp686I2288,MGC44911
  • Gene Description:
  • calcium/calmodulin-dependent protein kinase II delta
  • Gene Summary:
  • The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a delta chain. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Distinct isoforms of this chain have different expression patterns
  • Other Designations:
  • CaM kinase II delta subunit,CaM-kinase II delta chain,CaMK-II delta subunit,OTTHUMP00000163829,OTTHUMP00000163830,calcium/calmodulin-dependent protein kinase (CaM kinase) II delta,calcium/calmodulin-dependent protein kinase type II delta chain
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