PLD2 (phospho Y169) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: TNF treated Jurkat cells, Lane 2: antigen-specific peptide treated Jurkat cells with PLD2 (phospho Y169) polyclonal antibody (Cat # PAB29251) at 1:500-1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain tissue by PLD2 (phospho Y169) polyclonal antibody (Cat # PAB29251) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human PLD2.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y169 of human PLD2.
Host
Rabbit
Theoretical MW (kDa)
95
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of PLD2 only when phosphorylated at tyrosine 169.
Form
Liquid
Purification
Affinity purification
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (without Mg2+ and Ca2+), (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: TNF treated Jurkat cells, Lane 2: antigen-specific peptide treated Jurkat cells with PLD2 (phospho Y169) polyclonal antibody (Cat # PAB29251) at 1:500-1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain tissue by PLD2 (phospho Y169) polyclonal antibody (Cat # PAB29251) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — PLD2
Entrez GeneID
5338Protein Accession#
O14939Gene Name
PLD2
Gene Alias
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Gene Description
phospholipase D2
Omim ID
602384Gene Ontology
HyperlinkGene Summary
Phosphatidylcholine (PC)-specific phospholipases D (PLDs; EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control.[supplied by OMIM
Other Designations
choline phosphatase 2
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Interactome
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Pathway
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Disease
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