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PLD2 (phospho Y169) polyclonal antibody

  • Catalog # : PAB29251
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human PLD2.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y169 of human PLD2.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 95
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody detects endogenous levels of PLD2 only when phosphorylated at tyrosine 169.
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Recommend Usage:
  • Immunohistochemistry (1:50-1:100)
    Western Blot (1:500-1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (without Mg2+ and Ca2+), (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: TNF treated Jurkat cells, Lane 2: antigen-specific peptide treated Jurkat cells with PLD2 (phospho Y169) polyclonal antibody (Cat # PAB29251) at 1:500-1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human brain tissue by PLD2 (phospho Y169) polyclonal antibody (Cat # PAB29251) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 5338
  • Gene Name:
  • PLD2
  • Gene Alias:
  • -
  • Gene Description:
  • phospholipase D2
  • Gene Summary:
  • Phosphatidylcholine (PC)-specific phospholipases D (PLDs; EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control.[supplied by OMIM
  • Other Designations:
  • choline phosphatase 2
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