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NCF1 (phospho S345) polyclonal antibody

  • Catalog # : PAB29250
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human NCF1.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S345 of human NCF1.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 45
  • Reactivity:
  • Human
  • Specificity:
  • This antibody detects endogenous levels of NCF1 only when phosphorylated at serine 345.
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Recommend Usage:
  • Immunohistochemistry (1:50-1:100)
    Western Blot (1:500-1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (without Mg2+ and Ca2+), (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: TNF treated HepG2 cells Lane 2: antigen-specific peptide treated HepG2 cells with NCF1 (phospho S345) polyclonal antibody (Cat # PAB29250) at 1:500-1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human brain tissue by NCF1 (phospho S345) polyclonal antibody (Cat # PAB29250) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Gene Name:
  • NCF1
  • Gene Alias:
  • FLJ79451,NCF1A,NOXO2,SH3PXD1A,p47phox
  • Gene Description:
  • neutrophil cytosolic factor 1
  • Gene Summary:
  • The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq
  • Other Designations:
  • NADPH oxidase organizer 2
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