DPYSL2 (phospho T509) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human DPYSL2.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T509 of human DPYSL2.
Host
Rabbit
Theoretical MW (kDa)
50
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of DPYSL2 only when phosphorylated at threonine 509.
Form
Liquid
Purification
Affinity purification
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (without Mg2+ and Ca2+), (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: heat shock treated HT-29 cells, Lane 2: antigen-specific peptide treated HT-29 cells with DPYSL2 (phospho T509) polyclonal antibody (Cat # PAB29239) at 1:500-1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain tissue by DPYSL2 (phospho T509) polyclonal antibody (Cat # PAB29239) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — DPYSL2
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Interactome
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Pathway
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Disease
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