Product Browser

Last updated: 2017/8/20

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

EFNB1/EFNB2/EFNB3 (phospho Y324) polyclonal antibody

  • Catalog # : PAB29232
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human EFNB1/EFNB2/EFNB3.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y324 of human EFNB1/EFNB2/EFNB3.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 46
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody detects endogenous levels of EFNB1/EFNB2/EFNB3 only when phosphorylated at tyrosine 324.
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Recommend Usage:
  • Immunohistochemistry (1:50-1:100)
    Western Blot (1:500-1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (without Mg2+ and Ca2+), (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: serum treated K562 cells, Lane 2: antigen-specific peptide treated K562 cells with EFNB1/EFNB2/EFNB3 (phospho Y324) polyclonal antibody (Cat # PAB29232) at 1:500-1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human brain tissue by EFNB1/EFNB2/EFNB3 (phospho Y324) polyclonal antibody (Cat # PAB29232) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 1947
  • Protein Accession#:
  • P98172;P52799;Q15768
  • Gene Name:
  • EFNB1
  • Gene Alias:
  • CFND,CFNS,EFL3,EPLG2,Elk-L,LERK2,MGC8782
  • Gene Description:
  • ephrin-B1
  • Gene Summary:
  • The protein encoded by this gene is a type I membrane protein and a ligand of Eph-related receptor tyrosine kinases. It may play a role in cell adhesion and function in the development or maintenance of the nervous system. [provided by RefSeq
  • Other Designations:
  • ELK ligand,OTTHUMP00000023456,eph-related receptor tyrosine kinase ligand 2,ligand of eph-related kinase 2
  • Gene Information
  • Entrez GeneID:
  • 1948
  • Protein Accession#:
  • P98172;P52799;Q15768
  • Gene Name:
  • EFNB2
  • Gene Alias:
  • EPLG5,HTKL,Htk-L,LERK5,MGC126226,MGC126227,MGC126228
  • Gene Description:
  • ephrin-B2
  • Gene Summary:
  • This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors. [provided by RefSeq
  • Other Designations:
  • HTK ligand,eph-related receptor tyrosine kinase ligand 5,ephrin B2,ligand of eph-related kinase 5
  • Gene Information
  • Entrez GeneID:
  • 1949
  • Protein Accession#:
  • P98172;P52799;Q15768
  • Gene Name:
  • EFNB3
  • Gene Alias:
  • EFL6,EPLG8,LERK8
  • Gene Description:
  • ephrin-B3
  • Gene Summary:
  • EFNB3, a member of the ephrin gene family, is important in brain development as well as in its maintenance. Moreover, since levels of EFNB3 expression were particularly high in several forebrain subregions compared to other brain subregions, it may play a pivotal role in forebrain function. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH Receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq
  • Other Designations:
  • Ephrin B3,eph-related receptor tyrosine kinase ligand 8
  • RSS
  • YouTube
  • Linkedin
  • Facebook