Western blot analysis of Lane 1: antigen-specific peptide treated HepG2 cells, Lane 2: 3T3 cells, Lane 3: HepG2 cells with PTK2B (phospho Y579) polyclonal antibody (Cat # PAB29229) at 1:500-1000 dilution.
Immunohistochemical staining of human brain tissue by PTK2B (phospho Y579) polyclonal antibody (Cat # PAB29229) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq