Product Browser

Last updated: 2017/7/23

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

ITCH (phospho Y420) polyclonal antibody

  • Catalog # : PAB29228
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of human ITCH.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y420 of human ITCH.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 102
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • This antibody detects endogenous levels of ITCH only when phosphorylated at tyrosine 420.
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Recommend Usage:
  • Immunohistochemistry (1:50-1:100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (without Mg2+ and Ca2+), (50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Avoid repeated freezing and thawing.
  • Applications
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human brain tissue by ITCH (phospho Y420) polyclonal antibody (Cat # PAB29228) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Gene Name:
  • ITCH
  • Gene Alias:
  • AIF4,AIP4,NAPP1,dJ468O1.1
  • Gene Description:
  • itchy E3 ubiquitin protein ligase homolog (mouse)
  • Gene Summary:
  • Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells. [provided by RefSeq
  • Other Designations:
  • NFE2-associated polypeptide 1,atrophin-1 interacting protein 4,dJ468O1.1 (atrophin 1 interacting protein 4 (AIP4)),itchy homolog E3 ubiquitin protein ligase,ubiquitin protein ligase ITCH
  • RSS
  • YouTube
  • Linkedin
  • Facebook